Freezing

Freezing - ETCHING (freeze etching) - a technique for preparing samples for examination by electron microscopy. Unfixed tissue is frozen; then it is split into separate layers, and the ice layer is cleared from the surface being examined. The resulting image does not contain distortions caused by chemical fixing agents.

ETCHING (eng. freeze etching from freeze - freeze, etch - etching) is a method of preparing samples for study by electron microscopy, in which the sample is not fixed and is quickly frozen. The sample is then split into layers, removing the layer of ice that forms on the surface of the sample. After this, the resulting sample can be examined using an electron microscope.

ETCHING produces an image of a sample without the distortion caused by the chemical fixatives typically used in preparing samples for electron microscopy. In addition, this method allows you to study living cells and tissues without destroying their structure. ETCHING is widely used in biology, medicine, materials science and other sciences.

However, it must be taken into account that ETCHING can damage the sample, so it must be carried out with caution and only under the supervision of experienced specialists. It is also necessary to select appropriate conditions for freezing and crushing the sample in order to obtain a high-quality image.

Freezing or etching is a method of preparing samples for research using electron microscopy, in which tissues of animal or plant origin are not fixed and are frozen in an ice chamber until tightly bound ice crystals form. After this, the tissue is split (peeled off) and cleaned