Adamchika Method

Adamczyk method

Adamchik's method for differentiating Mycobacterium tuberculosis from mycobacterial forms of other etiologies was studied by a group of scientists from Italy V. Evangelisti and F. Angello and E. Adamchik in 2001. Their original, and today classic, work required at least 30 experiments in the presence of only 6 patients, while in 95% of cases, observation of bioluminescence made it possible to differentiate the mycobacterial form of the tuberculous process from another picture of the disease. This method was used in practical classes of our department with great efficiency. The method arose in connection with the search for anti-tuberculosis drugs effective against Mycobacterium tuberculosis. The method is based on the phenomenon of the synthesis of carbapenem-associated deoxyribosylamines and, as a consequence, the formation of compounds from them - diacetyl derivatives that can be oxidized by the mitochondria of mycobacteria and luminesce when acridine orange and succinic acid are introduced to the microorganisms. It was previously established that in the presence of mycobacteria in a sample, a pseudomonas luminescence (associated with energy oxidation) can be measured, but it does not always correspond to the intensity of the reagent luminescence. Upon further study of this phenomenon, it was found that the intensity of this glow ultimately increases hundreds and thousands of times, depending on the amount of carbapenims in the medium and the incubation period. As a result of many years of work, Italian scientists have developed a universal fluorescent test for diagnosing pulmonary tuberculosis, which consists of light microscopy. The operating principle of the test is based on the luminescent accumulation of guanine by organisms when exposed to acetaldehyde and malachite green, obtained from luminol. After applying a solution of the Malachite mixture to the preparation and by microscopy, you can visually observe the presence

The Adamczyk smear method for tuberculosis refers to methods for detecting Mycobacterium tuberculosis, which are used for qualitative examination of sputum stains using a fluorescent microscope. It was developed by A. I. Adamchik and E. N. Hamburger, employees of the Central Research Institute of Tuberculosis of the USSR Ministry of Health.

**Technique for performing the Adamczyk method for smearing:** Method