Kumona Method

Kumona method

Colorimetric method for determining indican content in urine

The Kumona method is a colorimetric method that is used to determine the levels of indicans in urine. This method is based on the ability of indicans to react with ninhydric acid, which forms colored products.

To carry out the analysis, you need to mix a urine sample with ninhydrin acid and leave it for some time. Then the sample must be treated with an acid solution and an indicator solution added. The result is a colored product, the intensity of which depends on the amount of indican in the sample.

This method is widely used in the clinical laboratory to diagnose diseases associated with metabolic disorders. It can also be used to monitor the health of people who suffer from alcoholism or drug addiction.

However, it should be noted that this method is not accurate and may give false results. Therefore, to obtain more accurate results, it is necessary to use other analysis methods, such as mass spectrometry or chromatography.

Kumona method

Urinalysis is one of the most important laboratory research methods that helps diagnose many diseases and pathological conditions. One of the main indicators that are checked during a urine test is the content of indican, a breakdown product of uric acid in the body. Determining the level of indican allows you to assess the state of metabolism and prescribe the correct treatment. There are various methods for determining indican, one of which is the Kumona (Kimura) method.

The **Kumona method** is a **colorimetric** method for determining the content of **indican** in urine, which is based on its reaction with **ninhydrin**. The essence of the method is that when urine is mixed with a solution of ninhydrin, a colored compound is formed, which is then measured using a photometer. Thus, kumona is a fairly fast and convenient way to determine indican and can be used in both clinical and scientific laboratories.

The technique for carrying out the kumon method is quite simple and does not require a large amount of reagents or special equipment. It begins by collecting the patient's urine and then adding a ninhydril solution to it. The resulting mixture is mixed and then placed in a dark place for several minutes to allow the effect to manifest. After this, the solution is filtered, and the resulting reaction products are measured photometrically. The exact level of indican is determined by comparing the color intensity of the resulting mixture with a predetermined calibration scale. Based on the results of the analysis, a conclusion is issued on the content of indican and the degree of metabolic disorders in the patient’s body.

The high sensitivity and accuracy of the Kumon method make it an indispensable tool in the diagnosis of various diseases. It can be used, for example, to analyze indican levels in urinary tract diseases, kidney diseases, diabetes, hypertension and other metabolic disorders. Kumon can also be used to monitor the effectiveness of treatment and determine the need to change the dosage of medications.