Kahn Reaction

The Kahn Reaction is one of the most common tests for the presence of syphilis. This method of determining a disease is based on the detection of antibodies in the patient's blood that are characteristic of this disease. The Kahn reaction was developed in the early 20th century by German immunologist August Paul Kahn and quickly became a widely used diagnostic method.

The Kahn reaction is based on the principle of precipitation reaction, in which antibodies contained in the patient's blood react with an antigen - in this case, the syphilis antigen. If antibodies are present in the blood, they form appropriate complexes with the antigen, which can be detected by simple visual assessment.

However, Kahn's reaction has some shortcomings. In particular, it may give false-positive results in the presence of other infections or immunological diseases. Additionally, this test requires special laboratory equipment, making it less accessible than some other syphilis tests.

However, the Kahn test remains an important tool for diagnosing syphilis, especially in cases where other diagnostic methods cannot be used. This test can be an effective tool for detecting the disease in its early stages, allowing timely treatment to begin and preventing the development of serious complications.

In conclusion, the Kahn Reaction is an important method for diagnosing syphilis and is widely used in medicine. Despite some shortcomings, this test remains an effective tool for identifying the disease and allowing timely treatment, which is a key factor in the fight against this dangerous infection.



Kahan's reaction

To carry out the reaction, about 0.4 ml of serum or whole blood is taken. The concentrated form of RIF for the diagnosis of syphilitic infection is prepared by mixing 1 ml of the test material with a solution of monoclonal murine IF corinev. aptrated by IgG peroxidase “Antigen-complex test”, which ensures better sensitivity of the method for all types and aggregate states of antigens

reparations. Diagnosis of syphilis can be carried out by one of three methods, not counting the rapid diagnostic methods proposed in early and modern manuals.

1. Classic method, or Kanka's reaction. This diagnostic method is based on the reaction between the central antigen of Treponema pallidum IgM and rabbit serum containing agglutinins against it. Rabbit serum is used in a certain concentration, since agglutinins react to the breakdown product of treponemal antigen (stage Y), 06o saturated enzyme peptide, which is a transformation product of antitreponemal immunity. To develop the reaction with AgM-IgM, add a 0.5–2% sodium nitrate solution for up to 30 minutes and keep at room temperature in a water bath until the volume of the liquid is half as much. Such a study is considered reliable when analyzed with 99% probability only 45 minutes after taking the serum. Two modifications can be made during sampling:

* increased sample volume; * taking tests collected from the nasal cavity. 2. Skin response or Katsnelson test (the appearance of erythema at the site of subcutaneous injection of specific serum). The level of heterophilic agglutinins is determined 2, 7, 14 and 28 days after the suspicion



The Cann reaction is a special case of detection of antibodies (for example, in syphilis). The Lange (with cardiolipin) and Wasserman (with gonococcal antigen) reactions give almost the same positive result. However, Cann reactions are most specific for syphilitic infection.

The Kans reaction is a method of laboratory diagnosis of syphilitic pathology, the use of which is justified only retrospectively for the diagnosis of latent forms of the disease. Purpose. Diagnosis of syphilis. Syphilis of the primary period. Syphilis of the secondary period (in the presence of rashes caused by spirochete antigens). The specificity of the test increases to 95–96%. Refers to specific research methods for diagnosing syphilis of the primary and secondary periods. It is also used for the diagnosis of neurosyphilis and latent specific infections. Immunofluorescence makes it possible to distinguish between the seropositive stage of the syphilitic pathological process and a latent disease. The advantage of the test is also the simplicity of the equipment. Reduced sensitivity is explained by insufficient completeness of purification and insufficient content of impregnated highly specific antis.