Wick Method

Knott method

Joseph Charles Knott is an American microbiologist, biochemist and immunologist. Born May 3, 1917 in New York. During the war he was drafted into the US Army, where he served in a medical research laboratory. After the war, he graduated from the College of Physicians and Surgeons (1946) and Harvard Medical School (1953). Then he did research at the Michigan Memory Institute of Bacteriology, and later headed laboratories at the Johns Hopkins Medical Center.

In 1968, he developed the first method of “intensive diagnostic procedures” - multiplex determination of antibodies based on data on their molecular specificity. This method is now called the Knott method. In the 70s of the 20th century, the concept of local subunits of the immune system was introduced, which were considered as a single adaptive response. He also separated local and general responses and identified the mechanisms of local and systemic responses. He also proposed polymerase chain reaction (PCR) technology, in which amplifiers speed up the process, and analysis of the results