Antron Method

The anthrone method is one of the methods for the quantitative determination of mono- and oligosaccharides. It is based on the ability of these compounds to produce furfural derivatives when heated in an acidic environment (for example, with sulfuric acid). These derivatives then react with anthrone (anthrone is an organic compound that is yellow and is used as a dye) to form a colored compound.

The anthrone method is widely used in biochemical and clinical diagnostics, as it allows you to quickly and accurately determine the amount of mono-, oligo- or polysaccharides in biological fluids (for example, in blood or urine). This is important for diagnosing various diseases associated with impaired carbohydrate metabolism, such as diabetes, as well as for monitoring the treatment of these diseases.

The procedure of the anthrone method is as follows: a sample of biological fluid (for example, blood or urine) is placed in a test tube, after which sulfuric acid is added and heat is added. Then anthrone is added to the resulting mixture, which forms a colored compound with furafural derivatives formed during heating. The color intensity of the resulting solution is measured using a photometer, and from this measurement the amount of mono-, oligo- or polysugars in the sample is calculated.

One of the advantages of the antron method is its high sensitivity and accuracy. It allows you to determine even very low concentrations of carbohydrates in biological fluids, which is important for diagnosing and monitoring the treatment of various diseases. In addition, this method is fast and easy to use, making it attractive for use in clinical practice.

However, like any other method, the antronic method has its limitations. For example, it may give false positive results in the presence of other compounds that can form colored compounds with anthrone. Also, some types of carbohydrates may not respond to this method, which can lead to false negative results.

Antron method

The anthrone (anthrone) method is a colorimetric technique for the detection of mono-, oligosaccharides and polysaccharides. It is based on the ability of the studied sugars to interact with an acidic sulfur mixture, as a result of which a furfural derivative is obtained, which is a dye in the presence of anthrone. This method is successfully used in biochemistry research and diagnostic analyses.

There are many reasons why this technique has gained such wide popularity:

This technique is used in various areas of clinical diagnostics, since at the moment there are many diseases associated with various types of diabetes. This is especially true for children. For example, this method can detect the presence of hidden diabetes mellitus in children, and also effectively conduct saliva tests. The colorimetric anthrone method is simple, cheap and widely available, which allows it to be widely used in practical work in laboratories.

"Anthrone method" for qualitative and quantitative analysis of mono-, disaccharide and polysaccharide compounds

– This is a reactive method of quantitative photocolorimetry based on the study of the absorption spectra of colored derivatives formed by the interaction of an anthrone reagent with polysaccharides under the action of concentrated sulfuric acid. The method is relatively simple, but requires expensive equipment. It is used both in the determination of monosaccharides in foods (for example, sucrose) and in the study of drugs, blood and urine.

One of the important advantages of the method is its versatility - the method is suitable for studying not only the polysaccharide content of starch, but also the polysaccharide fraction of tissues. This makes it possible to characterize the methods of structure of protein molecules and macromolecular material containing a large number of polysaccharides. In this regard, the use of the method for the study of biopolymers is of great interest.