Kokkel Method

The Kockel method is a method of staining histological preparations, which was developed by the German forensic physician Robert Kockel (R. Kockel) in 1865. It is used to detect fibrin, which is a protein formed in the blood when blood vessels are damaged.

To color the preparations, Coclell used a mixture of 1% chromic acid, hematoxylin dye and 10% potassium alum. After treating the preparations with Weigert's mixture, fibrin turned dark brown or dark blue.

The Coclell method is one of the most common methods for detecting fibrin. However, it has some disadvantages. Firstly, this method does not always give accurate results, since fibrin can be colored differently depending on its concentration in the preparation. Secondly, treatment of slides with Weigert's mixture may result in tissue damage, which may hamper analysis.

In addition, the Coclell method is not always convenient for use in everyday practice. It requires special reagents and equipment, and also takes a lot of time and effort. In this regard, many laboratories use simpler and more accessible methods for staining preparations.

However, despite its shortcomings, the Coclell method remains an important tool in forensic science and other fields where tissue analysis is required. It allows the rapid and accurate detection of fibrin in histological samples, which helps in the diagnosis of many diseases and injuries.

Kokkel's method Fibrin in tissues.

Kokkel is a method for detecting fibrin during microscopic examination of various tissues. Name Introduced into the clinic by the doctor Kockel (1857) to explain the coloring of the drug with a crystalline sulfur preparation according to the Schallmit method. There is the I) kokkel method of coloring histiosalics with bile with the addition of an alcohol-camphor mixture: in this case, fibrin appears in a bright orange color, and chromatic acid gives a blue-green color, bile gives a green color to the nuclei; then the preparations pass under hemetidine, which gives a violet-blue color around the nuclei and bright in