Lowry Method

Lowry method

The Lowry method is a method for determining protein content in biological samples, which was developed by the American pharmacologist O. N. Lowry in 1941. This method is one of the most common and accurate methods for determining protein content and is used in various fields of science and medicine, such as biology, chemistry, medicine, pharmacology, etc.

The essence of the method is as follows: a sample of biological material is placed in a solution with a certain amount of dye (usually biuret dye), which binds to the proteins of the sample. The sample is then treated with an acid or alkali to break the bonds between the dye and the proteins. The remaining dye is measured spectrophotometrically, allowing the amount of dye bound and therefore the amount of protein in the sample to be determined.

One of the advantages of the Lowry method is its high accuracy and reproducibility of results. In addition, this method can be used to determine the content of various types of proteins, such as albumins, globulins, enzymes, etc. The Lowry method is widely used in scientific research and clinical practice for diagnosing various diseases associated with protein metabolism disorders.

Lowry method

The Lowry method is a cytochemical method that allows one to evaluate the dynamics of oncotransduction of cell DNA by determining the melanin content in cells. It was developed in the 60s by physician Lawrence J. Lowry. This method allows you to determine the degree of malignancy of the tumor and carry out therapy for malignant diseases.

**Method description**

To carry out the cytochemical method, the cell membrane is examined. To do this, the membrane is treated with the following substances:

1. 2,7-dichlorodiethanol: this solution gives a blue color to the membrane; 2. quercetin; 3. HRP hematein.

The ratio of the drug to dichlorodiaminoethylene solution is 1:10,000