Staub-Traugotta Trial

The Staub-Traugott Test is a combined method for determining the sensitivity of bacteria to antibiotics. This method was developed in 1953 by Swiss pharmacologists Stauba and Traugott.

The essence of the method is that several different concentrations of the antibiotic are applied to the surface of a special plate. Colonies of bacteria are then placed on this surface to be tested for sensitivity to this antibiotic. If bacteria grow on the surface of the plate, this means that they are sensitive to this antibiotic.

The Staub-Traugott test is one of the most common methods for determining the sensitivity of microorganisms to antibiotics in medical practice. It allows you to quickly and accurately determine which antibiotic will be most effective for treating a specific disease.

However, like any other method, the Staub-Traugott test has its drawbacks. For example, some bacteria may be resistant to certain antibiotics, which may result in treatment failure. In addition, this method does not allow us to determine what specific mechanisms of bacterial resistance to antibiotics exist.

Despite this, the Staub-Traugott test remains one of the most important methods for determining the sensitivity of microorganisms to antibiotics and continues to be used in medical practice to this day.

The Staub-Traugott test is a diagnostic method that is used in microbiology and bacteriology to determine the presence of nitrogen gas in the blood. This test is used to monitor nitrogen-containing anaerobic bacteria that can cause infections such as abscesses, peritonitis, infective endocarditis and others.

The history of the Staub-Traugott test goes back to 1930. One of the authors of the method, German physician Ernst Strauss, used it to treat liver abscess caused by streptococcal infection. The test was used to check for the presence of nitrogen gas, allowing doctors to determine the presence of infection and prescribe the correct treatment.

However, the Staub-Traugott test was named after the Swiss immunologist and microbiologist Othmar Staub and the German internist and pathologist Alexander Traugott, who described and standardized the method for measuring nitrogen diphases in a blood sample more than 20 years after its first use.

The test is performed as follows:

The concentration of the K2HPO4 solution (dibasic potassium phosphate) is set. The blood being tested is being prepared. The test paper used is graph paper divided into 0.5 millimeter squares (0.04 grams), which is pre-colored red. Five milligrams of blood is placed on test paper and dried for 7 minutes. The paper is then dropped into a cylinder where different solutions are tested. Next, a solution of Na2S2O4 and NaHCO3 is added to change the pH level to 7.4. Cylinder-shaped beakers containing KOH solution are used. When the pH level exceeds 7.4, the test is completed. The condition of the gas cartridge, which indicates a positive result, means the presence of nitrogenous compounds and gas. For mass testing of one blood sample, racks for five samples can be used. To repeat the study, it is necessary to use new material. The shelf life is no more than a day.