Vignal-Veyon Method

Vignal-Veylon method - (W. Vignal, French bacteriologist of the 19th century; A. Veillon, 1864-1931, French bacteriologist)

The Vignal-Veylon method is used to stain bacteria. It was proposed by French bacteriologists of the late 19th and early 20th centuries V. Vignal and A. Veillon.

The essence of the method is to fix smears with bacteria with 96% ethyl alcohol, then stain with carbol fuchsin and decolorize with 1% hydrochloric acid. This allows acid-fast bacteria to be identified as red against a background of discolored acid-labile bacteria.

The Vignal-Veylon method has been widely used to identify acid-fast mycobacteria, in particular the causative agent of tuberculosis. It is still used in microbiology laboratories.

The Veillon vignalation method is one of the most effective methods for determining bacteria in various environments. This method is used to test food and water safety. It is based on the use of thiocyanates, which help identify bacteria and other microorganisms.

Vignalation and Veillon methods are the result of scientific research by French scientists Vignalle, Cottrip and Veillon. In 1913, these scientists discovered that when certain salts or acids were added to water containing bacteria, the liquid changed color. This color change was due to the formation of sediment or paint on the surface of the glass.

This method was named Vignal after Vignal's work published in the "Journal des sciencies." The Vignal method uses several types of liquids and salts to identify different types of microorganisms.

Over time this