Autoradiography Sledovaya

Autoradiography is a research method that allows you to study the structure and properties of materials at the atomic level. One of the options for autoradiography is track autoradiography, which allows you to count the number of tracks - and - particles formed as a result of the interaction of particles with the surface of a material.

Trace autoradiography is carried out as follows: a microscope equipped with a special device for recording i-particles is placed on the surface of the photographic material. The material is then irradiated with - or - particles, which interact with the atoms of the material and form tracks. Under a microscope, you can observe the number of tracks formed as a result of irradiation.

This method is widely used in science and technology to study the properties of materials, such as strength, hardness, electrical conductivity, etc. In addition, trace autoradiography can be used to determine the composition of materials and determine their structure.

Thus, trace autoradiography is an important method for studying materials, which allows one to obtain information about the properties of materials at the atomic level and determine their composition and structure.

Autoradiography traces for the article ----

Trace autoradiography is a method for studying the microstructure of cells, tissues and organs by counting tracks of γ- and β-emitting particles. In this case, the molecules and atoms that were damaged by particles and decay products of radioactive elements are counted. Therefore, this is both radiographic and chemical analysis at the same time. Damage to DNA molecules was previously the only possible mechanism for how radiation could have a positive effect on a tumor. Research has shown that the real reason is somewhat more complex: sometimes only the presence of viable radiosensitive molecules in cells can lead to tumor growth or control. But even if the concentration of molecules decreases to a level at which exposure to radiation will lead to cell death rather than stimulation, irradiation of cells will still lead to the breakdown of RNA and other molecules that are responsible for protein synthesis. This can cause an increase in cell division. Modern clinical trials no longer recommend the use of high doses of radiation in the treatment of tumors that are classified as low radiosensitive (type 1) or moderately radiosensitive (type 2), but for higher doses of radiation (several Gy) there is usually a need for special methods of delivering radiation to avoid damage to normal cells. [1] In laboratories, a high concentration is used from 3 thousand to 10 million culture cells in 24 ml of liquid, while when analyzing tissue, the concentrations are much lower and amount, respectively, to 50 - 75 thousand / ml.