Kullberg-Malyarevsky Method

The Kullberg-Malyarevskaya method is a method for determining the activity of transaminases in blood serum, proposed by Soviet biochemists L.M. Kullberg and M.E. Malyarevskaya.

The essence of the method is to determine the rate of pyruvate formation as a result of transamination of alanine or aspartate. In this case, specific substrates are used: L-alanine + α-ketoglutarate (for determining ALT) or L-aspartate + α-ketoglutarate (for determining AST). The resulting pyruvate reacts with 2,4-dinitrophenylhydrazine to form a colored compound, the amount of which is measured photometrically.

The method is characterized by high sensitivity and specificity. It allows you to separately determine the activity of ALT and AST in blood serum, which is important for the differential diagnosis of liver diseases. The method is widely used in clinical biochemistry.

Kullberg-Malyarevsky method

The Kulberg-Malyarevsky method is a method for determining the protein content in biological fluids and tissues, developed by Soviet biochemists L. M. Kulberg and M. E. Malyarevskaya. The method is based on the reaction between proteins and the dye bromophenol blue and subsequent measurement of the color intensity of the solution.

The principle of the method is that proteins in solution interact with the dye, forming colored complexes. The intensity of the color depends on the amount of protein in the solution. A photometer or spectrophotometer is used to measure color intensity.

To carry out the analysis, it is necessary to prepare a protein solution, add a dye and measure the color intensity. The instrument is then calibrated using standard protein solutions of known concentration. After this, the sample can be analyzed.

The Kullberg-Malyarevsky method is widely used in biochemistry and medicine to determine the content of proteins in blood serum, urine, cerebrospinal fluid and other biological fluids. The method is also used in the food industry to control the quality of food products.