Degreasing in Histological Technique

Defatting (demastification) is the process of removing fatty components from tissue during the preparation of histological preparations for microscopic examination. For this purpose, special chemical reagents are used that dissolve lipids and remove them from the tissue.

Degreasing is an important stage of histological technique, as it allows one to obtain better and more informative microslides for research. Without this process, false positive results may be obtained, especially when examining tissues containing large amounts of lipids, such as brain, liver, heart, kidneys, etc.

The degreasing process can be done manually or using automatic systems. In the first case, the tissue is placed in a solution of chloroform or alcohol, which dissolves lipids. The fabric is then washed with distilled water and dried.

When using automatic systems, degreasing occurs in special chambers where the fabric is treated with chemical reagents. After this, the fabric is washed and dried.

Although automatic degreasing systems are more convenient and efficient, the manual method also has its advantages. For example, it allows you to more precisely control the process and obtain more accurate results. Additionally, the manual method can be used for small tissue samples that are not suitable for automatic cameras.

In general, degreasing is an important stage of histological technique, which allows one to obtain more informative and accurate microslides. Regardless of which method is used, it is important to follow all rules and procedures in order to obtain quality research results.

Lipid removal is the partial or complete removal of lipids, oils, fats or waxes from biological material to alter or improve the quality of its processing (smear on a glass or slide, fixation and staining). The most common methods for obtaining fat-free drugs: transfer of lipid substances (in the form of a layer, film or floating drops) to sediment