Schultze Nadi-Oxidase Reaction

The Schultze-Nadi-oxidase (SN) reaction is a method for the detection and quantification of glycogen in tissues and cells. This method was developed in the 1930s by German pathologist Walter Schultz.

The essence of the method is that when an iodine solution containing hydrogen peroxide is added to a tissue or cell, glycogen is oxidized to glucose. This produces a blue-colored solution. The intensity of the color depends on the amount of glycogen in the sample.

The SN reaction is widely used in biochemistry and medicine for the diagnosis of various diseases associated with impaired carbohydrate metabolism. It can also be used to determine muscle and liver glycogen levels.

Thus, the SN reaction is an important method in biochemistry, which allows you to quickly and accurately determine the amount of glycogen in various tissues and cells of the body.

The Schultze-Nady reaction is a clinical diagnostic study in which deoxyribonuclease and DNase H are determined in human urine to determine inflammatory diseases in the genitourinary system. This method is very important in medicine, since inflammatory damage to the organs of the urinary-excretory system can have a wide variety of symptoms and manifestations, and its timely diagnosis is often necessary.

There is also a coprogram that contains a similar technique - determining the activity of pancreatic elastase-1 in feces. This enzyme is a marker of damage to the pancreas, because it is the pancreas that produces this enzyme, which is most present in this organ.

As for the biochemistry of urine, a group of enzymes and proteins that are actively involved in the transformation of foreign substances and food metabolism is studied here. Urea and creatinine, which are waste products, are also determined in the urine. Thus, urine biochemistry is needed to determine the functional functioning of the kidneys, the state of the genitourinary system, and the liver.