Levin's Nutrient Medium

Levin's culture medium Introduction

The nutrient medium (MS) was created in 1887 by the Russian physiologist Alexander Petrovich Chekalov (Chekalov A.P.). The PS received the name Levin nutrient medium due to the surname of Chekalov’s son, Vasily Petrovich Levin, who was engaged in scientific work in the field of microbiology. Later, in the 20s of the 20th century, thanks to the work of Sergei Ivanovich Vinogradsky, a metallurgical engineer, more accurate descriptions of this nutrient medium appeared. In the middle of the 20th century, a method was proposed for cultivating microbial cells in closed test tubes without adding foreign substances, which made it possible to preserve the viability and functions of microorganisms. The cultivation method using PS is currently considered classical, and PS itself is considered universal. The use of PS for the cultivation of various microorganisms (bacteria, fungi, viruses, etc.) has not only significantly accelerated and simplified various processes of microbiological synthesis, but also greatly reduced the cost of feeding bacteria and fungi with nutrient media during mass laboratory work.

Description of the method The method of cultivating microorganisms using PS allows the cultivation of microorganisms in narrow hermetically sealed tubes (biological fluids - BF) made of various materials. At the same time, the medium does not contain solid substrates for nutrition, which can be replaced with various substitute solutions, which is of great importance both for the preparation of standard model media for comparison, and for obtaining media with a specific function. Closed-volume BZs create conditions for artificial cultivation and allow for various experiments to study the dynamics of microorganism growth or their interaction. In the future, for ease of understanding, we will call these tubes test tubes or flasks. Currently, numerous types of PS have been created. It is believed that the fewer components contained in the PS (usually water, organic and inorganic components), the more