Dogel Method

Dogel's method of staining chromatin for the differentiation of animal oocytes and some plant tissues (bleaching the nucleus or protoplasm for 5 minutes with citrate alcohol) along with Balbi (bk). A. S. Dogel (1949) proposed treatment with cold water and snow during an alkaline reaction. D. m. - addition of coloring f. Schulze-Hingel "Nitropurpur" solution for the detection of deoxyribonucleic acid (DNA) or in an acidic environment with acidic hematein cyanine 8S, but more sensitive. In combination with Teichmann's mordant, the coloring is specific (D.N. Ushakov, 1961), recommended for differentiating metaphases in immature eggs of chicken waterfowl and hedgehogs, as well as eggs of many ungulates. D. m. - a histochemical staining option - DNA dyes with hemoglobic and riboflavin, when intense blue staining caused by riboflavin residues fluoresces under a microscope and is observed only in selected interphase nuclei (metaphases II - III, III - IV). D.M. serves to control inhibitors of procambial activity. The awakening of the normal spindle and 42 chromosomes is easily determined when growing human epithelium in a vivarium (under conditions of light isolation). When this material is incubated after 44 hours of incubation period after removal of cytostatics from the medium, the most firmly preserved chromosomes are blocked at early interkinesis, which is manifested by scattered fluorescent glow of the cytoplasm of cells in phases O and II.