M-Nadi-Oxidase Reaction

The m-nadi-oxidase reaction (also known as the stable nadi-oxidase reaction or Schultze nadi-oxidase reaction) is a biochemical test used to detect the activity of the m-nadi-oxidase enzyme.

This enzyme catalyzes the oxidation of NADH (the reduced form of nicotinamide adenine dinucleotide) to NAD+ (the oxidized form) with the help of molecular oxygen. The reaction occurs as follows:

NADH + H+ + O2 → NAD+ + H2O

During the test, tissue or extract samples are incubated in the presence of NADH. If the sample contains active m-nadi-oxidase, then NADH will be oxidized to NAD+, which can be determined by a decrease in light absorption at a wavelength of 340 nm.

Thus, the amount of NADH oxidation can be used to quantify the activity of m-nadioxidase in the sample under study. This test is widely used in biochemical research to study the metabolism, respiratory chain and other processes associated with this enzyme.

SYN. Nadi oxidase reaction. Schultz staining option. A simplified version may be a color defect resulting from the oxidation of the main component of hemoglobin - heme. M. is easily accelerated in the presence of iron ions.

Synonyms - nadi - oxidase (stable) reaction (N.O.S.), shultz-naidu - oxidase reaction (CNUR). The coloring agent is hydrogen peroxide; the indicator dye is metanitrosamine (methylene blue, indole), the substrate is serum blood. Mainly used for rapid diagnosis of anemia when acute hemolysis is suspected and identification of unstained hemoglobin S (recessive polymorphism). The method is based on the oxidation of hemoglobin to form carbonylhemoglobin; combines with methylene blue (indole) to form a blue-violet colored compound. The test fluid (usually 95% peroxide and salicylic aldehyde solution) turns the blood serum blue