The rapid development of biotechnology and the emergence of new methods for studying living organisms require the creation of convenient and diverse media for the cultivation of microorganisms. One of the modern approaches to creating nutrient media involves the use of nanotechnology. Nanoparticles in culture media can perform a variety of roles, from improving cell growth to increasing the specificity of cell interaction with antigens. A culture medium is a medium that contains all the necessary elements for the growth of living organisms (such as bacteria or yeast), and allows them to grow to a certain stage (optimal population). The purpose of creating a nutrient medium is to provide a sufficient amount of nutrients to support the vital functions of organisms and the optimal temperature for their growth and development. The main substances of the nutrient medium are glucose and dextrose; they serve as a source of energy for microorganisms. In addition, culture media contain other substances such as amino acids, vitamins, hormones and antibiotics. One of the widely used types of nutrient media is media created according to the Karl Frenzel method (Frenkel nutrient media, Frenzel's medium, or simply French medium). Microbiologist Karl Frenzel was famous for his research in the field of infections and chemotherapy. He developed the first derivative of the artificial cultivation medium for microbial cultures, "French". In his research, he experimented with different physicochemical environmental conditions and found that microbes can grow even in highly contaminated substrates under strictly defined conditions. An important characteristic of the French medium was its accessibility to all types of microorganisms, regardless of their morphology.
The main advantage of the Friznchik nutrient medium is that it contains an increased concentration of carbon dioxide, oxygen and other important elements for the aerobic growth of microorganisms. It also has a lower osmolality compared to other media, making it an ideal choice for culturing not only pathogens.