Diphenylamine Reaction

The diphenylamine test, also known as the diphenylamine test (DPA test), is a chemical test that is used to determine the presence of organic acids in biological fluids.

Diphenylamine is an organic compound that, when reacted with organic acids, forms colored products. When these products enter a liquid, they cause a color change that can be observed using special instruments.

To carry out the diphenylamine reaction, it is necessary to mix a small amount of the test liquid with diphenylamine and leave for several minutes. Then add a small amount of alkali to stop the reaction and measure the color change.

Depending on what organic acids are present in the liquid, the color can vary from yellow to red or purple. This method is used in medicine to diagnose various diseases such as diabetes, liver and kidney diseases.

Despite the fact that the diphenylamine reaction is a simple and accessible method for the determination of organic acids, it cannot completely replace more accurate analytical methods. However, due to its simplicity and accessibility, this method can be useful as an additional tool for diagnosing diseases.

**Diphenylamine reaction** is a color chemical titrimetric and volumetric direct or inverse qualitative determination associated with the chemical reaction occurring between aromatic amines and diphenylamine or between a diphenylamine reagent and an aromatic amine. When an aromatic amine interacts with diphenylase, the ammonium ion H+ is split off, forming a secondary aromatic amine (sulfo-N-DiPA), which is colored blue.

It follows that the distinctive qualities from other probe reactions are: * Separation of anion diffusion peaks or their masking, since the reagents can interact with each other; * Characteristic color of the secondary reaction product to evaluate the result of the analysis; * Reaction mechanism of interaction of ions (electrostatic interactions); * Formation of a complex from several components (dye + anion). Schemes of the structures of primary reagents and colored compounds obtained from their use associated with the same classes of reagents (redox reagents) can be easily seen in the figure - presented below.

Diphenyl disulfide is a molecular complex of sulfomethyl acid anions (CH3SO2 -H) and secondary aromatics or alkaloid metabolites. In differential colorimetric reactions of diphenyl diacetate with dopamine-S-oxidase and dopamine decarboxylase, it not only turns well into blue or turquoise (425 nm), but does not even lose its color after many chemical transformations (subject to storage conditions). The tannin-based reagent produces a pink color saturation (using the original reagent) with a blue edge. The manifestation of the diphenyl reaction in all cases is preceded by the approach of the anion and the diphenant molecule in an aqueous solution, holding them together with the electrostatic effect [1] equally and with symmetrical or modified formation of covalent bonds, [2] due to the alkylation of the diphenyl atoms of the aromatic rings with carboxylic acids with the deprotonation of atomic groups sulfur and the formation of complexone or adducts [3]. Here the definitions are valid according to the formula of the cluster complex: Fe 2 O 3 (CH 3 COO) 6; BaSO 4-K/FeOH or cluster formula in the form of a flat octahedron, with mutual penetration of oxygen and sulfur atoms.

Diphenylamine reaction - (synonym: diphenylamine reaction, DFA reaction)

The diphenylamine reaction is a diphenamine modification that has a general appearance, is characterized by many similarities, and is used in pharmaceutical, anthrone and other research. For this purpose