Yolk medium (also known as McCoy-Chapin medium) is a nutrient medium used in microbiology for the cultivation of microorganisms. It was developed in 1941 by James McCoy and Edward Chapin and named after them.
Yolk medium is a nutrient medium containing egg yolk, which provides a source of nutrients for microorganisms. In addition, it contains various additives such as peptone, glucose, yeast extract and other components that provide essential nutrients for the growth of microorganisms.
A wide range of microorganisms, including bacteria, fungi and protozoa, can be cultured in the yolk medium. It is also used to determine the sensitivity of microorganisms to antibiotics and other drugs.
However, it should be noted that the yolk medium can be toxic to some microorganisms and can lead to their death. Therefore, when using yolk medium, certain precautions must be taken, such as using sterile instruments and equipment.
Despite some shortcomings, yolk medium remains an important tool in microbiology and continues to be used in research and clinical practice.