Gelber's Nutrient Medium

Currently, the use of various types of nutrient media for the cultivation of microorganisms has a wide range and is one of the most popular methods in microbiology. One of the most common media is Gelber's nutrient medium. It was invented more than 150 years ago and is named after the German doctor and scientist Hermann Gelberg.

Description of Gelber's nutrient medium This medium was originally developed for storing bacterial cultures, but later it began to be used as the main element for growing living microorganisms. This solution has high nutritional value for living cells of microorganisms, due to its composition and component content. Components of the nutrient medium Compared to many other media, the gelber nutrient medium contains components such as sugar, gelatin, hydrochloric acid and yeast extract. Sugar provides energy support for microbial cells, and yeast extract is a source of amino acids and other nutrients. Gelatin is the only component that gives the medium its name. It gives it the necessary viscosity and allows it to keep bacteria alive and active for a long time. Purpose and use Considering the positive viability of microorganisms in Gelber's medium, it is actively used in such fields as medicine and bacteriology. The medium is also an integral part of many research works in biology and microbiology, such as enzyme analysis, bacterial metabolism, etc. **Gelbert's nutrient medium** Gelzer's medium (Muller-Hinton broth) is a balanced nutrient broth containing salts and buffer systems. The main feature of this broth is the presence of proteolytic enzymes. This nutrient medium is ideal for the cultivation of cytotoxic strains of _Enterobacteriaceae_, for example, Salmonella spp. or _Serratia_ spp. Also, with Helbert's medium, _Vibrio cholerae_ (biovar cholera), species _Yersinia, Bordetella, Proteus mirabilis_ can be cultivated on solid media. Mueller-Hinton broth can also be used in the analysis of Escherichia coli. Undoubtedly, this occurs due to the ability of this colony to form a specific amino sugar ring. When added to an Agar agar block with the organism Hektoenomyces, kale correlates with _Klebsiella oxytoca_ or _Proteus vulgaris._ When Aga cyclate is inoculated with polysaccharide, a characteristic "bluish" colony of _Shewanella putrefaciens_ appears, which is characterized by luminescent features. However, _Flavobacterium meningosepticum_ can be differentiated from marine vibrios by adding liquid media to agglutinating colonies. Virulent bacteria most often do not grow on broth media and agglutinins can only enter the agar medium. Therefore, for use in these cases, Mueller-Hinton broth can be diluted with Aga culture. If we are dealing with phages, it is necessary to carry out analyzes of specific fecal antigens or polysaccharides. After inoculating these cultures in Mueller-Hinton broth, after incubation the microbes microbes