Kruger Method

The Kruger method is a method of analyzing genetic data developed by American bacteriologist Arthur P. Krueger in the 1940s. The method is based on the analysis of DNA polymorphisms and allows us to determine the genetic structure of the population.

The Kruger method is widely used in human, animal and plant genetics to study genetic diversity and evolution of populations. It is also used in medical genetics to diagnose hereditary diseases and assess the risk of their development.

The method is based on the analysis of DNA polymorphism, which represents differences in the nucleotide sequence that can be found in the genome. The Kruger method allows you to determine the number and location of polymorphisms in a population, which allows you to establish genetic similarities between different groups of individuals.

To carry out the analysis, special markers are used, which are short sections of DNA containing polymorphisms. Markers are identified using molecular genetic methods such as PCR (polymerase chain reaction) or DNA sequencing.

After identifying markers, their distribution in the population is analyzed. This analysis can be done in a variety of ways, such as statistical methods or maximum likelihood.

The use of the Kruger method allows one to obtain important information about the genetic structure of a population and its evolution, as well as the inheritance of various diseases.

Kruger method The Kruger method is a classic method for determining antibiotics, especially the generic resistance of pathogenic microorganisms to penicillins, tetracyclines and some other groups of antibiotics. This is also a method of testing for membrane markers, as it uses solubilization of various components of the staphylococcal membrane with solutions of different protein concentrations. The bacterial suspension is pre-enriched with various groups of antibiotics and thermostatically incubated at 37 °C for a certain time (usually 24 hours). Then the same solutions of antibiotics dissolved in ethanol are added to the test tube with the seed suspension and left for several days at 4 °C. Different groups of antibiotics will cause different amounts of particle dissolution (solubilization) of different levels of membranes, turning the solution into different colors. The evolution of the method was observed and developed by two American scientists - Frederick Copper and Friedrich Lexer at a meeting of the American Chemical Society in 1889. The method has such a term as the MIC value.