Kunitza Method

Kunitz method

Kunitze is an enzyme that breaks down a non-protein toxin during poisoning. The enzyme was discovered in the thirties of the last century by the American researcher Edith Masako Kunitz. Hence, the method is named after her. Kunintze is the first enzyme discovered through genetic engineering.

Kunitz method: it is based on the use of electrophoresis and methylene blue - a substance that, when the pH changes, breaks down into aquasol blue and cunitine hydrochloride. Cunitine hydrazone turns polyelectrolytes blue. The reagents used are harmless. The peculiarity of the method lies in its simplicity, in which the coloring of protein targets occurs as proteins are added to the electrophosphate solution, and not at the time of its separation after electrophoresis. The method was developed in 2014 by a team of Russian scientists living in Tomsk, already in 217.