Zeissler's Nutrient Medium

**Zeissler's nutrient medium** is one of the microbiological methods for determining living microorganisms in milk. When adding 3% peptone to 20% fat acetic dibutyl ether (patent of M.I. Zhelikhovsky) to 1 ml of milk, digestion of lactose under the influence of cymatic acid salts present in milk does not occur, but fusel acid can be released. Due to the presence of sugar, the medium has vitamin activity due to the induction of biotin synthesis. When fatty acids and alcohol enter the environment during the day, aminodihydrophthalazine - indoxyl is formed, which with the amine groups of hemoglobin proteins in the blood