Sterilization Cold

Cold sterilization (lat. sterilisatio frigida) is a sterilization method based on the use of low temperatures. With this method, microorganisms die or lose their ability to reproduce as a result of freezing.

Cold sterilization is most often carried out using liquid nitrogen or dry ice (solid carbon dioxide) at a temperature of -70-80°C. Such low temperatures cause the formation of ice crystals in the cells of microorganisms, which leads to their death.

The advantages of the method are the absence of chemical residues and the negative effects of high temperatures. However, cold sterilization has limited use because not all materials and equipment can withstand such low temperatures. The method is effective for sterilization of thermolabile substances and materials.