Jaffe Reaction

Jaffe Reaction

Synonyms: safranin test; McLecalin's test.

A method for detecting sugar in urine based on the principle of the reaction of polysaccharides with black Sudan (diastase). Urine into a test tube saturated with sulfuric acid (to prevent an enzymatic reaction), then 10 ml of aprotic mazein and heated. If there are carbohydrates in the sample, a red precipitate will form after 20 minutes. Add 5 drops of a 50% aqueous solution to the sediment